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In this paper, polyamidoamine dendrimers were cross-linked to polyamide microfiber synthetic leather base pretreated with sulfuric acid by using glutaraldehyde and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide as crosslinking agents to improve the hygienic properties of the base. Water vapor permeability and moisture absorption were the main performance indicators in this process. The SEM and AFM images of the modified base show that the microfiber pores moderately opened and the fiber became rougher. Contact angle and energy dispersive spectrometer analyses reveal that the hydrophilicity of the modified microfiber base were enhanced and the active groups increased. In addition, the hygienic property of the modified microfiber base significantly improved, and its physical properties relatively increased.