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The Surface enhanced fluorescence (SEF) and Surface Enhanced Raman Scattering (SERS) of?Au nanoparticle films deposited on Si and SiO2 substrates are presented. From the experimental?results, it is concluded that the fluorescence peak intensity changes in a similar way with the Raman?intensity for the various substrates. Both the fluorescence and the Raman intensity were much?stronger on SiO2 substrate than on the Si substrate. That is due to the Crystal Violet (CV) adsorbed?on the substrate having different refractive index effect the electrical field near the nanoparticles.The nanoparticle size effect on the Raman and fluorescence was also studied.