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An antifouling coating was prepared with the polyaniline(PANi) as conductive filler, chlorine ether resin as film forming material, and methylsulfonic acid as doping acid as well as other additives. The influences of PANi content on the coating’s conductivity, physical properties and chemical resistant performance were studied. The electrochemical behavior of the electrode painted by the coating was investigated by Tafel analysis and electrochemical impedance spectroscopy measurements. Results showed conductive properties were significantly influenced by the mass ratio of doping acid/PANi, and when the mass ration of doped methylsulfonic acid/PANi was 1:1, surface resistance obtained a lowest value. Conductive properties, chemical substances resistance and anti-corrosion properties of conductive coatings were all affected by the concentration of PANi added: the concentration of PANi added in the coating should be controlled in the range from 15% to 25% range, where it had both perfect conductive property and unique corrosion property. Electrolysis tests showed that an electrode made by the conductive coating could produce a certain concentration of HOCl, so as to achieve the anti-fouling purpose.