Ru(DMSO)4Cl2 is a good precursor complex in synthetic inorganic chemistry and also considered to be an efficient anti-cancer drug. Here in, we are reporting ～80?nm nano-aggregates of Ru(DMSO)4Cl2 complex stabilized Nafion membrane modified glassy carbon electrode (GCE/Nf–{RuDMSO–Cl–H2O}–MME) for simultaneous electro-catalytic oxidations and separation-less detection of purine bases; uric acid (UA), xanthine (X) and hypoxanthine (Hx) in a physiological solution. The MME was characterized using solid state UV–Vis, X-ray photoelectron spectroscopy (XPS) and transmission electron-microscopy (TEM) techniques. It has been found that Ru(DMSO)4Cl2 undergoes dissolution reactions within the water rich hydrophilic micro-channels of Nafion membrane and get electro-statically stabilized as [RuIIClx(DMSO)y(H2O)4?(x+y)]n+ through the cationic sulphonic acid structures. The MME film behaves like metallic electronic conductor, investigated with [Math Processing Error] redox couple. This new electrode shows efficient electrochemical sensing of the UA, X and Hx with a calibration windows (detection limit) up to 700 (0.372?μM), 500 (2.351?μM) and 300?μM (2.37?μM) respectively. The electrode shows exceptional stability and applicability to various real samples.
Annamalai Senthil Kumar,and Puchakayala Swetha.
Journal of Electroanalytical Chemistry,642,2,135-142(2010)